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技術(shù)文章

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當(dāng)前位置:首頁技術(shù)文章重組小鼠神經(jīng)膠質(zhì)細(xì)胞衍化營養(yǎng)因子GDNF

重組小鼠神經(jīng)膠質(zhì)細(xì)胞衍化營養(yǎng)因子GDNF

更新時(shí)間:2013-06-17點(diǎn)擊次數(shù):1269

Synonyms :

Glial-Derived Neurotrophic Factor, ATF-1

Description :

GDNF is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFR α(α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Catalog #:

450-44

Source :

E.coli

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted GDNF is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Biological Activity :

The ED50 was determined by the proliferation of rat C6 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

AA Sequence :

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI

PubMed Link :

mouse+AND+GDNF

Country of Origin :

USA

 

 

Size A 2µg $75   
 
Size B 10µg $185   
 
Size C 1mg $5200   

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